| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3211 | Leptospira biflexa | GCGATCGAAGCGTTGAACC | TTTGCCTTGTTTGTCGCGT | 59.94 | 59.20 | 213 | 45 |
100.00%
|
100.00%
|
| Primer 3212 | Leptospira biflexa | AACTCCTCCACCCACTCGT | TCGGCTTTGTCCATCCCA | 60.15 | 58.51 | 199 | 45 |
100.00%
|
100.00%
|
| Primer 3213 | Leptospira biflexa | CGCCAACTGTGCTGCATT | ACAACCGTGAACTCCGCA | 59.35 | 59.50 | 208 | 45 |
100.00%
|
100.00%
|
| Primer 3214 | Leptospira biflexa | ACGAAGCCCAATTCCCGT | TGGCCTGCCAACCTTCAA | 59.25 | 59.39 | 162 | 45 |
100.00%
|
100.00%
|
| Primer 3215 | Leptospira biflexa | CGGCACCAAACAAGCGTT | TTGTGAGTTCAGGAAGGCGT | 59.59 | 59.53 | 233 | 45 |
100.00%
|
100.00%
|
| Primer 3216 | Leptospira biflexa | GGTGCAGTCCAGGCTCAAT | TGCCGAACACCAAATCCGT | 60.00 | 60.23 | 259 | 45 |
100.00%
|
100.00%
|
| Primer 3217 | Leptospira biflexa | GTTGCAAAACGAGCGCTCT | TGGCCAAGTGCTCGGATT | 59.72 | 59.24 | 152 | 45 |
100.00%
|
100.00%
|
| Primer 3218 | Leptospira biflexa | TACAGCGCATGGGGTGTT | TACGCCAAGCCACATCGT | 59.25 | 59.34 | 282 | 45 |
100.00%
|
100.00%
|
| Primer 3219 | Leptospira biflexa | CGCGATCCGACGAGTCATT | ACGGGTTCTTGCGTTGGT | 60.30 | 59.81 | 169 | 45 |
100.00%
|
100.00%
|
| Primer 3220 | Leptospira biflexa | AGCATGCCCACAAAGACGT | TGCGACGTTTGTCCACAC | 60.23 | 58.60 | 260 | 45 |
100.00%
|
100.00%
|